A threshold value of 2% was observed to produce the most consistent volume measurements (judging by the lowest variation in final volume of distribution measured for different subjects) and thus was chosen as the threshold value for the data shown here.
Of all the environments, CAP stored around 3°C and sheltered from light yielded the most consistent absorbance measurements for all three concentrations tested.
For each experiment, the time course of ethidium fluorescence was measured for 20 isolated cells and then averaged to obtain the mean fluorescent signal, the slope of the fluorescent signal versus the time was used as the most accurate and consistent measurement for comparisons.
Of nine preprocessing algorithms tested, PLIER+16 produced expression values that were most consistent with RT-PCR measurements, although the difference in performance between most of the algorithms was not statistically significant.
We found that GFP as a reporter, combined with a drug selection marker, gave the most consistent and convenient mutation rate measurements.
Quantitation of autophagic flux by flow cytometry can be accomplished by other means, but we have found that using the ratio of mCherry to GFP fluorescence is the most consistent, robust, useful, and least arbitrary measurement of flux we have tested.
In agreement with our results, interprotomer distances derived from fluorescence resonance energy transfer measurements are also most consistent with the 1M6N interface.
Hip flex at 120°/second and back extension at 30°/second were the two most consistent predictor variables for all BMD measurements across all age groups.
Reported half-times of recovery for H1 vary considerably, and our measurements in egg cytoplasm are most consistent with half-times on the order of seconds, not minutes [10].
This region of intersection corresponds to those parameter values most consistent with the parallel plaque and viral yield experimental measurements for a particular strain.
