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RPS 18 was the most consistent gene, and this was then used for the further analysis.
In contrast, GGEA, which takes consistency into account for selecting relevant gene sets in the first place, yields the most consistent gene sets in all categories for both, KEGG and GO gene set definitions.
The probe sets with the most consistent gene changes in the three cell lines represented 24 down-regulated and 12 up-regulated genes, all with closely related human homologues.
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Because Id2 had shown the most consistent rhythmic gene expression in all cell lines and tissues, we generated transgenic mice lacking the entire coding region and therefore null for the Id2 gene and examined their circadian systems.
The most consistent differences in gene expression between myd88−/− and wild-type embryos were observed for transcription factors and genes involved in signal transduction.
Some success has been demonstrated in reducing the problem to the most consistent sequence-verified gene annotations between two platforms (e.g. UniGene cluster membership) and examining correlations, ratio values, or gene calls, although sensitivity and global statistical inference of such approaches still remains a challenge [ 7, 10- 12].
Most investigators using microarrays focus on the largest and most consistent differences in gene expression.
Correspondence between human psoriasis and mouse phenotypes was also evident from inspection of the "trademark" expression patterns of human psoriasis, which we identified as the most consistent and pronounced gene expression differences between psoriatic plaques and normal human skin (Figure 5).
This is due to the fact that most of the consistent gene regulations in Sts can be detected by both strategies.
Collectively, the data from qRT-PCR of specific RNAs and from microarrays are most consistent with a gene expression signature for early-occurring tumors, regardless of histology.
One of the most consistent differences in gene expression between secondary progressive (SP-MS) patients and healthy controls was enhancement of HIF-1α and its downstream components [ 58].
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