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The PE-PPE protein class, while not well characterized, represented the third most abundant category and showed the most consistent expression during the infection.
This set includes genes exhibiting the most consistent expression change (over 2-fold) in cancer versus control tissues, which were deemed to be differentially expressed genes with high reliability, derived through multiple statistical tests.
Candidate genes identified from microarray studies alone are typically those with the highest or most consistent expression differences.
Of these GAPC was found to have the most consistent expression and was used as a control for normalizing differential transcription efficiency and RNA amounts.
The 20 HKGs with the highest and most consistent expression (See Methods) were selected from this list as the best candidates to serve as reference HKGs in laboratory experiments.
Our preliminary experiments demonstrated that out of all cell surface markers (OX-40L, CD30L, c-kit, CD127) reported to be upregulated on the human pro-inflammatory subgroup of ILCs called LTi cells, the stem cell growth factor receptor, otherwise called tyrosine protein kinase kit (c-kit; CD117) has most consistent expression on the proportion of ILCs in the blood and CSF.
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Nr4a1-eGFP expression is most consistent with expression in direct pathway striosomal neurons but a small proportion of met-enkephalin expressing cells also express eGFP in vitro.
Interestingly, the top-ranking gene in this group is CYR61 (Cysteine-rich, angiogenic inducer, 61; EntrezGene ID: 3491), which was identified in the original study as one of the seven genes with the most consistent differential expression between colon cancer and control samples [35].
Although Cyp2f2 displayed the most consistent differential expression pattern, it is unlikely that Cyp2f2 would have a unique role in mammalian aging apart from other cytochrome P450 enzymes.
The pattern was most consistent with endothelial expression, and expression was most marked in viremic patients.
A 1910 issue of The Craftsman said that in the Prior house, "we see a most complete and consistent expression of the Craftsman idea".
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