With regards to the most conserved ancient plant WOX proteins, the expression and function of AtWOX10 is unknown.
Genes responsible for terms like basic metabolic processes related to biosynthesis and degradation, transcriptional and translational activity, protein synthesis and destination and signal transduction are amongst the most conserved in plants [ 48].
Phase (3) is probably the most conserved part of the pathway across land plants.
Rbx1, an ubiquitin ligase, was the most conserved gene in plant DRR pathways.
Most conserved miRNAs in plants have been identified by traditional Sanger sequencing or computational approaches [ 43- 47].
Our results have proved that SlGRAS24 and SlGRAS40 can be cleaved by miR171 (Fig. 5), one of the most conserved miRNAs in plants, suggesting that they may have similar functions with their homologous genes characterized in other species such as Arabidopsis [ 43].
Therefore, since Glycine is known to be important in or near the EH domain of the animal and mammalian EHDs [19], [38], we examined the homology between the EH domain of all plant ESTs available, and chose to mutate the most conserved Glycine across all plant species available in the databases within the EH domain, thereby generating AtEHD2_G37R.
Finally, the general suppression of codon GTA was one of the most conserved features among the plant species we investigated.
Among these, 17 cloned miRNAs seem to be species-specific for Physcomitrella whereas the remaining eight miRNAs most likely represent new members of conserved plant miRNA families (Table 1).
