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Overall, the ACT1 gene was the most conserved locus (Table 6).
The most conserved locus is the STX1b locus, which emerged before the LCA of Euteleostomi, as it is found in both teleost fish and tetrapods.
The most conserved locus was rbcL with pairwise distances among Agalinis species ranging from 0 – 3.07% and averaging 1.07%; psbA- trnH had the largest range of among-species pairwise maximum likelihood distance of all the cpDNA loci, ranging from 0 – 20.50% and averaging 7.75% (Table 2).
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These were inspected by eye in the genome browser (UCSC) [ 48] and the most conserved loci (120 cases), representing the broad activity range of CR1 subtypes as compiled by the TinT method, were chosen to generate conserved PCR primers for amplification of orthologous loci in representative galliforms (see Methods for species sampling).
Disease resistance genes, including NBS-LRR genes, constitute the most conserved group of PHAS loci, confirming and expanding previous reports [ 15, 16, 23].
The 3' end of the TRA/D locus appears to be the most conserved across species since many of the loci have conserved synteny in both mammals and birds.
We identified the most conserved regions in the sex locus and designed primers for three key regions: the high mobility group (HMG) gene regions, the triose-phosphate transporter (TPT) region, and the RNA helicase region.
All V-regions (V1 - V9; [ 40]) of the 16S rRNA gene were free of introns, indicating that many introns are confined to the most highly-conserved loci in the 16S rRNA gene.
The consensus-degenerated hybrid oligonucleotide primer (CODEHOP) strategy was modified to design primers targeting the most conserved motifs in the RNA-dependent RNA polymerase locus.
To accommodate both of these variables an initial reaction set is performed using the most conserved primers, those representing the 16 S rrn locus (primer set 19; Table 1).
Most conserved miRNAs exists as gene families and are therefore represented by multiple loci in Arabidopsis, rice and Populus whereas recently evolved/evolving miRNAs are found to have a single locus in the genome [ 24- 26].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com