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We selected proteins that are not previously described in OA chondrocytes and are among the most confidently identified proteins (show very high frequency of identification between all samples and have high number of spectra in the label-free quantification).
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On the other hand, using λ=0.75 can most confidently identify the pathways regulating regulation of mitotic cell cycle.
Most proteins up-regulated in the CCII-lines were confidently identified based on LC-MS/MS sequence data (Table 1; Additional file 1).
This lack of nuclear orthologs could be due to two reasons: (1) these proteins are predominantly encoded by mitochondrial genomes such as most of the genes involved in oxidative phosophorylation, (2) these proteins are too diverged to be confidently identified by using MitoCOGs as queries (e.g. ribosomal proteins).
Most of the protein spots represented low abundant proteins with too small quantities of proteins on the 2D gels to be confidently identified, resulting in 37 successfully identified proteins (Table 2).
The first such object confidently identified as Martian was reported in 1983.
Thus, currently the molecule is not confidently identified.
In 2012, proteins were confidently identified for the first time, collected from a 43,000-year-old woolly mammoth.
Many potential novel signaling proteins and associated signaling pathways were confidently identified.
Other species were confidently identified to genus or higher taxon level.
The number of miRNAs confidently identified in the human genome is currently over 700 (703 in miRBase v13.0).
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CEO of Professional Science Editing for Scientists @ prosciediting.com