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To compare the same number of sections for each sample, the ROI dataset of every examined sample was reduced to the 70 most central sections.
Normally the two most central sections from each implant were chosen for analysis, and buccal and lingual surfaces were measured separately.
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Using a magnification of 20×, the histomorphometric analysis of each calvaria cap was performed on the most central section and on four adjacent sections.
Second, 3D CBCT results were calculated as an average value of different VOIs around the implant, while 2D histological results came from the most central section plane of the bone samples.
For each animal, new bone formation (NBF) values were calculated by the arithmetic mean of three most central histological sections of the calvaria, and another section was used for histological analysis.
For each animal, the values of NBF and RPA were represented by the arithmetic mean of the four most central histological sections of the calvaria.
The specimen sections were evaluated at the most central saggital section of each implant under an optical microscope after staining.
The most central sagittal histological sections (30 40 μm thick) were obtained using a Leica SP 1600 saw microtome.
Actually, the middle sections of most central pipes (if not all) are empty due to thermal expansion and contraction, and sometimes the central pipes at the tips are also empty by TEM observations (we have not observed the whole central pipes filled by the black substances), i.e., the Ni-containing substances in the central pipes are disconnected.
Three serial sections in the most central cut area, corresponding to the greatest dimensions of the samples in length and diameter, were selected and stained with the trichrome Masson stain.
In the next sub-section we analyze the most central 20 genes in more detail.
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