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Notably, most cells stained for EpCAM, CD44, and vimentin (Figure 2B), suggesting that EpCAM/CD44 signals underestimate the phenotypic diversity within early passage ovarian cancer cells.
In general, most cells stained positively for mIDH1R132 in tumours that harboured the mutated IDH1 R132H protein.
Muscle tissue sections were scored: -, no staining; +, few of the cells positively stained; ++, some (less than one-half) of the cells stained; +++, approximately one-half of the cells stained; ++++, more than one-half of the cells stained; +++++, most cells stained.
The following system was used to score the distribution of positive cells: none (not stained)=0, focal (less than one-third of cells stained)=1, multifocal (less than two-thirds of cells stained)=2, and diffuse (most cells stained)=3.
TRAIL+cisplatin co-treatment resulted in almost complete disappearance of Ki67-positive melanoma cells from the peripheral sub-population of skin reconstruct-embedded melanoma spheroids, while, concomitantly, most cells stained positive for cleaved caspase-3 and TUNEL.
After exposure to TiO2 NPs and PM10 for 24 h, fluorescence from most cells stained with H2DCFDA indicated that intracellular H2O2 had accumulated strongly in HUVEC; however, this was significantly inhibited reaching almost basal levels by pretreatment with DHEA at all concentrations used.
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Analyses of the resulting samples established that most cells staining positive for RPA were also EdU positive (Fig. 1C).
The weighted score is obtained by multiplying the staining intensity score (3+, strong positive stain in most cells; 2+, moderate stain in cells; 1+, weak stain in cells; 0, no evidence of stain) and score for the percentage of positive cells (3+, most of cells stained; 2+, half of cells stained; 1+, few cells stained; 0, no cells stained).
Even though the antibodies used to detect leukocytes in some of these studies have themselves overlapping specificities, a recent publication using a mouse model of liver injury showed that most FSP1-positive cells stained positively for F4/80, which is at present the most specific marker for macrophages [ 21].
In most sections, inflammatory cells stained positive for BLT2 and this provided a valuable internal control.
Interestingly, most of the cells stained with these antibodies were found to be TUNEL positive (88% of the revC14-positive cells), indicating that DNA degradation occurred in the active caspase-14-expressing differentiated keratinocytes.
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