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Most cells show nuclear staining with a T-Ag-specific antibody (Fig. 5b).
Analysis of a larger field of view containing many cells over a longer time confirmed that, whereas the majority of cells do not exhibit fluorescence foci, most cells show a focus at some point during the time sequence.
At high levels of induction (50 μM, Figure 4F), cells contain a high abundance of mRNA in the cytosol, and most cells show an active transcription site in the nucleus.
In vivo experiments, recording in the DG in animals with reduced neurogenesis show that they have reduced number of neurons with spiking broadly tuned to theta cycle, and most cells show a narrow tuning to a specific phase of theta, suggesting that immature cells could presumably contribute to the broadly tuned population.
Using previously reported criteria (Yoshikawa et al, 1999; Angèle et al, 2000), we compared the tumours to the adjacent normal epithelium (in which most cells show positive staining for ATM and BRCA1) and classified those tumours with more than 25% of ATM- or BRCA1-negative tumour cells as showing reduced expression of these proteins, all others being classed as showing normal expression.
In the cc2d2a−/− siblings, this punctate localization is observed in only 26% of expressing cells, whereas most cells show diffuse expression throughout the cytoplasm with occasional weak concentration in the inner segment (brackets in Fig. 7B and B′, quantification in Fig. 7C), indicating that Cc2d2a is at least partially required for punctate localization of Rab8 in the inner segment.
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Upon day 12, most cells showed neuronal morphology (Fig. 3B).
Most cells showed multiple striking peaks; later peaks had gradually decreasing amplitudes but were clearly discernible.
In the presence of blocking antibodies, most cells showed control levels of both transcripts.
Importantly, most cells showing features of this late autophagic cell death showed peripheral chromatin condensation in their nuclei, a hallmark of apoptosis.
Infected cells plus supernatants were freeze-thawed when most cells showed cytopathic effects (4 5 days post inoculation with 0.001 p.f.u./cell).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com