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Expression of pluripotency markers was highest and most consistent at the edge of the colony, where most cells expressed between 3 5 of the pluripotency markers shown (Figure 2b c).
In Ker-CT-Ras epithelium there was little or no co-localization staining, suggesting that most cells expressed either keratin-14 or involucrin but not both (Fig. 6, left center).
In contrast, in OP explants cultured in the presence of DAPT (50 µM), the generation of Hes5+ and Ngn1+ cells was blocked and most cells expressed HuC/D (Fig. 4G), characteristic of post-mitotic neurons.
By flow cytometry, most cells expressed de novo CD34 and upregulated CD31 within 1 week.
Most cells expressed a low HMGB1 mRNA labeling at all time points.
NCLSCs could also generate neurons, especially after growth with a mixture of neurotrophic factors, when most cells expressed neurofilaments and a minority expressed immunoreactive TRPV1 indicating sensory neurons.
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However, since the RD genes were expressed within just a handful of expression domains and most cells expressing TGFβ and Hh appear resistant to transformation into retinal tissue, the mechanism that may permit and restrict ectopic formation remains unclear.
In colon cancer, several immunohistochemical studies reported that cancer cells had TP expression [ 39, 40], while other studies reported that most cells expressing TP were stromal cells, especially macrophages and lymphocytes [ 41, 42].
In the HEK 293T cells, most cells expressing βB11 142 and βB11 173 did not contain protein aggregates, while about 45% cells expressing βB11 229 with a GFP tag at the N-terminus contained p62-negative aggregates (Figs. 1F and S6).
The Oct4 staining demonstrated that most cells expressing the signaling molecules were undifferentiated mES cells.
Mia1p-MutNES4-GFP was depleted from kinetochores in most cells expressing wild type Alp14p-TagRFP (Fig. 5B, upper panel, and 5C).
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