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NSC viability following PPy(DBS) reduction was poor and most cells detached from the polymer.
At higher concentrations (0.1 1 μg/ml), most cells detached from the culture dish, although the few cells that remained attached were almost all SA- βgal-positive.
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One representative movie corresponding to experiments evaluated in Fig. 4 a,b showing that perfused PMNs arrest on the apical surface of the normoxic BBB monolayer and start crawling in the direction of the flow, with most cells detaching during the 20 min acquisition time.
As a positive control, most of the cells detached by trypsinization of the long-term cultures from neonatal mouse brains –expected to be macroglia– were found to be strongly immunostained for GFAP (not shown).
Then, 1.5-2 mL trypsinizing solution was added to cover the flask surface, which was then left at room temperature for 3 min until most of the cells detached.
Most cells had detached after 24 hours.
However, recording reliable images of live cells turned out to be very difficult for two reasons: (i) most cells were detached or pushed aside during scanning; (ii) strong interactions between the tip and the soft bacterial surface led to fuzzy images that were difficult to interpret.
Most control siRNA-transfected cells detached from their neighbours and migrated directionally to fill the gap exposed as the result of the scratch wound.
Many Schwann cells detached from degenerating axons (Fig. 11E, arrow) and showed anomalous, most probably pre-apoptotic phenotype.
The functionalities of the cells detached by photoreaction were evaluated.
Planktonic cells (a), cells detached from biofilm grown on polycarbonate (b), cells detached from biofilms grown on stainless steel (c).
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