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In most cases, samples with calculated values greater than the cutoff values were omitted as outliers.
In most cases, samples with high anti-Neu5Gc IgG concentration also exhibited relatively high IgM concentration.
We do not have formal disease activity measurements for the patients with these other diseases but in most cases, samples were taken when patients were symptomatic.
However, we need to stress the fact that the effect of collectors and species could not be differentiated since in most cases samples from one species were collected by a single person under unique conditions (Additional file 1: Table S1).
Assuming that in most cases samples were taken a few days before to just after the death of the host, we think these dates represent acceptable, though not ideal, estimates of the endpoint of strain time.
In most cases, samples contained few multinucleated cells, amounting to less than 1% of the nevus cell population, although some nevi showed a higher abundance of these abnormal cells, reaching up to 23%.
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In most cases, sample sizes have been small and the study population highly selected and non-representative of the general population.
In most cases, sampling was carried out with an aspirator device or a funnel with an attached gauze bag, into which beetles where shaken from the plants.
Private haplotypes could also represent haplotypes that went undetected in other sky islands due to limited sampling, but we believe this is unlikely because in most cases sample sizes were high relative to the number of haplotypes.
The unsupervised clustering heatmap of all samples) was generated using Spearman correlations, and the results showed that most normal samples and most case samples were, respectively, clustered together, when all genera were used for clustering.
Remarkably, most normal samples (78%) were clustered in the N-like group, and most case samples (81%) were clustered in the OB-like group (Fisher's P value = 1.61E − 07).
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