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They were then repeated with the single African strain with the most base calls.
The analyses were first repeated with data from c167.4 (when available) and the Madagascar strain with the most base calls.
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Eighty percent of the sequence reads in the WGA-treated specimens had a Phred quality score of at least 20 (data not shown), suggesting that most base-calling had an accuracy of 99% [31].
When reporting uncertainty for bases called, most base-calling implementations relies on Phred scores rather than IUPAC encoding used by Rolexa [ 17].
After the rape of a teenage girl by three marines in 1995, the United States agreed to shut its most unpopular base, called Futenma, as soon as an alternative runway could be built.
This likelihood was approximated using the equation p = (1-pbinom(nMax,n,0.5)), 1-pbinom nMaxng the number of unambiguous base calls and nMax equaling the abundance of the most common base call.
Everyone on base calls him Sam.
The base call with the most reads is considered the primary base call.
For all variable sites, the base call with the most mapped reads was selected as the consensus using an in-house Python script.
In particular, most types of sequencing hardware provide a quality score Qb for each base call in the read.
Base call accuracy for most of the reads (92.6%) reached a threshold of 99% (i.e., the probability of a base call error of ≤ 0.01; Q20.01
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com