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The phylogenetic tree showed a concordance with the facts mentioned above, where the position of the Negritos on the tree were placed at the most basal position among the Malay, Indonesian and Chinese populations.
casei is placed at the most basal position, followed by Lb. salivarius.
In fact, Unknown 1 attains the most basal position in our PHYRN-NJ tree.
By contrast, Gastrophrynoides occupied the most basal position of the Australian-New Guinean subfamily Asterophryinae.
The C. elegans fat-5, fat-6, and fat-7 are placed at the most basal position of the tree.
A second hypothesis, supported by molecular data, puts Afrotheria in the most basal position [ 6, 10- 14].
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The 36-OTU dataset comprised only frogs, with Ascaphus and Leiopelma, which occupy the most basal positions among extant anurans (e.g., [ 58]), used as outgroups.
In terms of the non-monophyly hypothesis of the Archaeplastida, it is reasonable to suggest that the gnd gene phylogeny may reflect the host cell phylogeny as recently resolved by a multiple slowly evolving nuclear gene phylogeny [ 19], which demonstrated the non-monophyly of Archaeplastida and the most basal positioning of red algae plus Excavata within the bikonts (Fig. 2B).
Although recent studies [ 7, 14] have provided robust support for the most basal lineage position of Allenopithecus, the relationships of the other three major clades (i.e. the arboreal Cercopithecus clade, the terrestrial clade, and Miopithecus) remain unclear.
Furthermore, we revealed that the genus Gastrophrynoides, which is only found in areas derived from the Eurasian landmass (Borneo and the Malay Peninsula), occupies the most basal phylogenetic position among asterophryines, and this taxon split from other asterophryine lineages during the Eocene (around 48 Ma).
The inclusion of previously unsampled taxa in our phylogeny also produced new insights into Arvicanthini phylogeny, most notably the basal position of Golunda, followed by the divergence of Oenomys then by the highly supported clade containing Stochomys and Hybomys.
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