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PIC analysis can be used to evaluate markers so that the most appropriate marker can be selected for genetic mapping and phylogenetic analysis (Anderson et al. 1993; Powell et al. 1996).
While still concerned that any adjustment for unmet need should be founded on robust epidemiological data, a measure of health expectancy has been seen as the most appropriate marker of unmet need in England [ 30].
Mitochondrial DNA is the most appropriate marker in this instance as the mutation rate is rapid enough to provide variability over the time scales in question, and recombination is very unusual, enabling straightforward interpretation of the patterns observed [ 21].
Finally, further research is needed to determine the most appropriate marker or combination of markers to assess SHS exposure, which may include more specific environmental and individual markers of exposure (e.g., airborne nicotine and cotinine in saliva) in addition to PM2.5 concentration.
Alternatively the Lifeact FAM combination might not be the most appropriate marker in terms of affinity for F-actin: the position and/or the nature of the fluorophore and/or the linker between the fluorophore and Lifeact might affect the interaction with F-actin resulting in poor visualisation of dynamic events in real time.
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Identifying the most appropriate markers to employ has been challenging.
Although there is some concordance among the results of different groups in the search for biomarker miRNAs, uncertainty remains as to which miRNAs are the most appropriate markers of disease.
Having established what constitutes disease modification through pharmacologic intervention, it was necessary for the group to identify the most appropriate markers of disease status that can be used to monitor this process.
The difficulties observed for LDHA and VIL1 are probably due to the fact that these genes belong to large gene families and therefore suggest that they may not be the most appropriate markers for a standardisation effort.
We anticipate our complete mitochondrial genome amplification methods to be applicable to other amphibians, helpful for identifying the most appropriate markers for differentiating species, populations and resolving phylogenies, a pressing need since amphibians are undergoing drastic global decline.
Thus, despite being commonly used (e.g., Cox et al. 2008), ribosomal proteins alone are probably not the most appropriate markers to reconstruct very ancient evolutionary events such as the root of the archaeal tree or the relationships among the three domains of Life.
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