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Modeltest 3.7 [ 39] was applied to determine the most appropriate evolutionary model for nucleotide substitution found in the sequences.
Phylogenetic trees were constructed after using a model test to pick the most appropriate evolutionary model for our analysis in the Mega 5.2 program [ 89].
We used Modeltest [ 88] with the standard Akaike Information Criterion AICC) to determine the simplest and most appropriate evolutionary model for our dataset.
After excluding gaps and the ambiguous sites, we used ProtTest 2.4 [ 70] to choose the most appropriate evolutionary model for our data set.
ProtTest [ 72] was used to select the most appropriate evolutionary model for ML inference (DBD JTT+G; CTD JTT+G; Aux/IAA JTT+G+F).
Hierarchical likelihood ratio tests led to the adoption of the General Time Reversible model with a proportion of invariant sites and gamma distributed rate heterogeneity (GTR+I+Γ) as the most appropriate evolutionary model for the molecular clock analyses.
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All final alignments had a norMD score > 0.6 and have been provided in Additional file 7. The most appropriate protein evolutionary model for each alignment was assessed using ModelGenerator v.85 [ 83].
The treatments with copepods or copepod-cues resulting in altered gene expression patterns can be considered as an outcome of the ongoing dialog between Alexandrium and its environment and the result of integrating and processing the received information into the most appropriate response selected through evolutionary processes.
We hypothesize that the ranking of genomes according to lengths of their genes is the most appropriate approach in revealing evolutionary driving factors.
The DM values remove the strong and intrinsic effects of protein abundance on noise, and are the most appropriate for the study of evolutionary constraints.
Overall, conclusions reached by recent studies of mammalian genomes suggest that AR currently represent the most appropriate neutral reference for molecular evolutionary analyses (Thomas et al. 2003; Lunter et al. 2006; Eőry et al. 2010; Pollard et al. 2010).
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