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For example, claiming that "most alignment times recorded here might be considered manageable for most purposes" seems to be little more than the author's unsupported opinion, based on a relatively tiny number of reads.
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Although aligning two sequences takes time proportional to the product of their lengths, most alignment-free methods run in linear time.
The length of this time window (quarter) was chosen to ensure that most alignments had ≫2 sequences.
Most alignments ranged from 70 82 amino acids.
In contrast, PAGAN/free and PaPaRa needed 3.3 and 4.7 min for the most time-consuming alignment.
All samples (unlabeled and labeled, control, and LPS-stimulated) were analyzed together via a single call to xcmsSet, which performs peak detection and sets up the data for further processing, including most importantly retention-time alignment.
Finally, the energy calculation was deactivated for these tests, as the focus was on the most time consuming part, the alignment.
This could crucially improve the runtime of multiple-alignment algorithms, as calculating guide trees becomes the most time-consuming step in progressive alignment if the number of sequences grows.
In CUSHAW2, the most time-consuming part of SE alignment is the selection of the best mapping region using the SW algorithm.
Most divergence time estimates are only negligibly different from analysis of much smaller nuclear or mitogenomic alignments [ 23].
Most times, actually.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com