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The G2/M DNA damage checkpoint and pyruvate metabolism were among the most affected pathways, and Cancer was the most likely disease associated to HDV replication.
Due to the large number of affected pathways we limited our systematic analyses to the 23 most affected pathways (q<0.10) (Table 1).
The most affected pathways related to known biological and toxicological functions in LESCs as determined by the IPA software.
IPA revealed that DDR, cell-cycle control of chromosomal replication and ATM signalling were among the most affected pathways.
However, at each stage of the early infection the most affected pathways differ significantly when comparing each condition with the previous one).
In the FAD in vitro exposure (Table 1), epithelium morphogenesis, mitochondrial transport and protein amino acid glycosylation were among the most affected pathways.
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The most affected pathway was "Defense response" (GO 0006952, p = 0.003).
The most affected pathway was "Negative regulation of cellular process" pathway (GO 0048523, p = 0.036).
The second most affected pathway was 'focal adhesion', which contained upregulated genes that mostly encode ECM proteins.
Using KEGG pathway analyses, we found that the most affected metabolic pathways were oxidative phosphorylation in group 1 (Additional file 10), and photosynthesis in group 2 (Additional file 11).
The most affected mitochondrial pathways are, ketone metabolic process, organic acid metabolic process, oxidation reduction, amino acid metabolic process, and nitrogen compound metabolic process.
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