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Among the nonspecific acyl-ACP substrates tested, hexanoyl-ACP (C6ACP) was the most active substrate with BmaI1.
Indeed, the most active substrate had Gly in the Z position, whereas Glu, Asp, and Gln displayed the lowest activities (2, panel a).
Similar(58)
We find that the most active substrates presented here, those having an N-terminal Arg to the acetylated lysine and with basic and aromatic residues favored at the C-terminal position relative to the acetylated lysine, do not mimic many of the known natural substrates, including histones.
This array also revealed that SIRT1 has a general preference for aromatic residues in the Z position, but this effect depends on the residue in the X position, with the most active substrates having either basic or hydrophobic residues in the X position.
Interestingly, we observed substrate inhibition only for precipitated apo-ACP and unprecipitated octanoyl-ACP (method "a" above), which was also the most-active substrate in this series.
Therefore the data suggest that ERGIC is the most active membrane substrate for LC3 lipidation.
This KDAC3 complex is most active toward substrates having aromatic residues in both the X and Z sites (2, panel d).
Medium-chain acyl-CoA dehydrogenase is most active with substrates from hexanoyl-CoA to dodecanoyl-CoA, whereas long-chain acyl-CoA dehydrogenase preferentially acts on octanoyl-CoA and longer-chain substrates [ 59].
For example, interfacially adsorbed derivative was the most active using simple substrates (ethyl butyrate) while PEI derivative was the most active hydrolysing ionic substrates (2-phenyl-2-butyroylacetic acid at pH 7) or methyl mandelate.
Therefore, this enzyme is least active when there is excess product in the cell, and most active when its substrates are plentiful.
In general, we found that the Tev-mTALENs with the longest I-TevI fragments were most active on the substrates with longer DNA spacers.
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