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As shown in both Tables 4 and 5, MDH2 was the most active gene in several similarity measurements.
The adolescence cerebrum has the most active gene expression, with expression of a large number of regulatory genes up-regulated and some crucial pathways activated.
Although four genes encoding a non-heme chloroperoxidase are present in the B. cenocepacia J2315 genome (BCAL0771, BCAM2106, BCAM2109, BCAS0079), overexpression was only observed for BCAL0771, indicating the ability of our microarray-based strategy to highlight the most active gene in Burkholderia genomes where multiple paralogous functional pathways exist [ 13].
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On polytene chromosomes, this modification is localized to euchromatic interbands which contain most active genes, and is mostly excluded from the chromocenter and other heterochromatic regions [43].
In Drosophila, the transcription of most active genes is halted [8] and a restricted set of heat-shock genes, many encoding molecular chaperones and including HSEs in their proximal promoter regions, are coordinately upregulated during or after heat-stress [9].
Such a direct interaction is apparently not the case for most active genes since they are not located at the NPCs.
In order to keep only the most active genes we applied a coefficient of variation filter with a threshold of 1 across the different conditions.
Directed and global chromatin immunoprecipitation analyses reveal an unprecedented promoter occupancy pattern: most active genes are bound by only TAFs 3 and 5 along with TBP, whereas the remaining active genes are bound by TBP and all six hESC TAFs.
Promoters of most active genes do not only initiate transcription of the coding sequence, but also a substantial amount of transcription of the antisense strand upstream of the transcription start site (TSS).
Genome-wide analysis of mH2A distribution indicates that it is depleted from most active genes (Changolkar et al, 2010) and enriched on repressed chromatin (Buschbeck et al, 2009; Gamble et al, 2010; Barzily-Rokni et al, 2011).
Cohesin also indirectly alters transcription of many active genes that don't bind cohesin, which may arise in part from its positive regulation of the myc gene (Rhodes et al. 2010; Schaaf et al. 2013a), which encodes a protein that directly stimulates transcription of most active genes (Lin et al. 2012; Nie et al. 2012).
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