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Bioassay-guided fractionation was done on the most active crude extracts.
Different solvent crude extracts were screened, and the most active crude extract was further subjected to differential bioguided fractionation.
This was done for the most active crude extracts i.e. active on at least two bacterial species, and the pure compound which was active following the disc diffusion test.
The results of MIC/MBC/MFC for the biofilms as well as the MBC/MFC MIC ratio of the most active crude oils and fractions are shown in Table 4.
HPLC-DAD qualitative analyses were performed to determine and compare the general profiling of the compounds present in the most active crude extract of methanol and its aqueous fraction using Agilent Technologies (Santa Clara, USA) attached to a computer system.
The most active crude fraction from the bee pollen was then further enriched for bioactive components by silica gel 60 quick and adsorption or Sephadex LH-20 size exclusion chromatography.
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The most active methanolic crude extract was subjected further to differential fractionation using n-hexane, dichloromethane, ethyl acetate, n-butanol, and water.
A total of five fractions which consisted of n-hexane, dichloromethane, ethyl acetate, n-butanol, and aqueous were obtained from the differential fractionation of the most active methanolic crude extract through in vitro screening.
The air-dried powdered leaves of M. oleifera (500 g) were extracted successively with different solvents consisting of methanol, ethanol, and water to determine the most active solvent crude extract in vitro (at least three times for each solvent until the extract was exhausted) with the help of a shaker (Lab Tech shaker, model; LSI-3016R, Korea) set at 25°C.
The most active contract for crude oil on the New York Mercantile Exchange has risen 11percentt so far this month.
Iraqi records, for example, show that Glencore, a Swiss-based trading company that was one of the most active purchasers of Iraqi crude, paid $3,222,780.70 in surcharges.
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