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Exact(7)
A total of 4,333 clones were screened on intact wheat straw (In-WS), and the 4 most active clones (>4CV) were selected, using the activity of Tx-Xyn-AF7-bearing clones as the base case for comparison.
The metabolically most active clones were identified by screening for high specific glucose uptake rates during ammonium starvation-induced stationary phase in deep-well microtiter plates.
In six of the seven most active clones a wild-type serine at position 83 had been replaced by threonine, a substitution known to alter the substrate chain length preference of Lipolase® variants.
The apparent most active clones were further subjected to microtiter fermentations (in 96 deep-well plates).
Consequently, it was decided to analyze the metagenomic fragments of the nine most active clones that stood out in the PCA analysis and those of nineteen other randomly selected clones.
Among the 120 hits, a set of 40 most active clones, which revealed larger halo zones on agar plates containing tributyrin (lipases/esterases (LIPES) relating hits) and cellulases (CMCases) or strong chromogenic signals of glycosidase (GLY) and dehalogenases (HAD), were chosen for further analysis (some representative hits are presented on Fig. 1).
Similar(53)
Analysis of 84 active clones revealed that they all expressed lipase activity, but with lower activities than that of a wild-type Lipolase®-producing clone.
As the RR was only 12%, the present study demonstrates that at relapse, after the most active at present first-line regimen-incorporating all most active agents-the emerging tumor cell clones are possibly resistant to any available further alternative treatment.
The most active enzymes, identified by hydrolysis of chromophoric sugar aglycones, were selected for further investigation; including gene cloning, protein expression, and preliminary enzyme characterization.
The most active walked.
The Philippines' dispute is the most active.
Related(20)
most vigorous clones
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