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The most abundant transcript uniquely expressed in LD has only 558 reads (PGSC0003DMG400026590) whereas the most abundant unique transcript under SD conditions has only 208 reads (PGSC0003DMG400016462).
Among four types of management regime, mowing sites (especially traditional verge meadows) had most abundant unique, rare species specially adapted to regular cutting.
Then the next most abundant unique sequence was used as a seed and the remainder were compared to the new seed, until all sequences had been analyzed.
The most abundant unique small RNAs were 21, 22, and 24 nt in length.
A list of top eight most abundant unique genes in each tissue is presented in Supplementary Table S4.
To establish a threshold for this criterion, we determined the percentage of reads corresponding the most abundant unique sequence respect to the total of reads spanning the junction.
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To characterize and compare the bronchial epithelial and cancer transcriptomes, we computated average normalized tag counts for BE (14 libraries), CIS (5 libraries), and invasive SCC (6 libraries) datasets, and subsequently selected the most abundant 300 unique tags from each dataset for analysis (Table S1).
A putative sequence with high similarity to SQE was found to comprise the most abundant 17 unique sequences (Table 4).
Why the 24-nt smRNAs are the most abundant class of unique sequences in seed tissues compared to germinating cotyledons and the vegetative stem and leaf tissues is intriguing.
Again, the three most abundant subsets are those unique to each of the conditions.
The most abundant classes of these unique clean reads were 21 24 nucleotides (nt), and the 24 nucleotides (nt) sequences were the most common.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com