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Sialic acids are the most abundant terminal monosaccharides on glycoproteins and glycolipids in eukaryotic cells.
GNE catalyzes two sequential steps in the biosynthetic pathway of sialic acid [8], the most abundant terminal monosaccharide on glycoconjugates of eukaryotic cells [9].
These are the first two steps of N-acetylneuraminic acid (Neu5Ac) biosynthesis [10], the most abundant terminal sialic acid of the eukaryote glycoconjugates.
N-acetyl-D-neuraminic acid (Neu5Ac) is the most abundant terminal carbohydrate in glycoconjugates (glycoproteins and glycolipids) and has great commercial value.
Consistent with the effect of the terminal segments that induced their retraction, the order at which the curve peaked was lower with MPP+, so this was the order at which the values became zero; this means the most abundant terminal neurites were located at order 2, and there were no dendrites at order 6.
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Similarly, Helitrons in the C. elegans genome were most abundant in the terminal regions of each chromosome, which are often in the heterochromatin state.
Synaptic vesicles have a diameter of ∼30 nm and are most abundant in the terminals.
U was the second most abundant residue at the 5' terminal position in most cases except in 24-nt sRNAs, in which A was present with a frequency comparable to or higher than U (Fig. 3A and Fig. S4).
Moreover, the second most abundant nucleotide at the 5' terminal position of grapevine 24-nt vd-sRNAs (the A residue) is preferentially found at the same position in the 24-nt sRNAs recruited by Arabidopsis AGO4, which acts in concert with DCL3, suggesting that 24-nt vd-sRNAs may enter the DNA methylation pathway.
To test this prediction further, we replaced position 69 with tyrosine, which has only an average frequency (0.88) at helix-capping sites, compared to 1.41 for threonine, which is the second most abundant residue at N-terminal helix caps.
High-resolution data were manually inspected using Qual Browser (Thermo Fisher Scientific) software for all masses, at abundances of 0.1% or above relative to the most abundant form, corresponding to N-terminal peptides and identified.
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