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The sequence length distribution of small RNA showed the highest abundance at 22 nt, indicating miRNA-like sequences were the most abundant sequences identified in the majority of samples, representing from 20 to 62% of total RNA sequence reads.
With more than a million copies scattered across the chromosomes, Alu elements, which run about 300 bases in length, are the most abundant sequences in the human genome.
Gram-negative Bacteriodetes were the second most abundant sequences identified for both fresh and dry manure samples.
Members of Bacteriodetes and Actinobacteria were the second most abundant sequences identified from dry and fresh manure samples, respectively.
Phylogenetic analysis of the 10 most abundant sequences indicates that members of Clostridia followed by Bacteroidia predominated in fresh manure (Figure 2).
We also used complex network methods to construct a non-redundant database of miRNA candidates by selecting the most abundant sequences as representative of each cluster.
Following the most recent update to miRBase, far more of the most abundant sequences in our library corresponded with the updated miRBase reference, pointing toward the latter explanation.
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First, sequences were sorted by decreasing abundance since the most abundant sequence is likely to be a correct sequence, while less common sequences may include artefacts due to sequencing errors or PCR artefacts.
For miR-21, the reference sequence was the most abundant sequence.
The most abundant sequence of each OTU was chosen as seed sequence.
Therefore, it was concluded that the most abundant sequence cluster represented putative clones of L. ochracea.
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