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The top ten of the most abundant sequenced candidates are listed in Table 3.
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First, sequences were sorted by decreasing abundance since the most abundant sequence is likely to be a correct sequence, while less common sequences may include artefacts due to sequencing errors or PCR artefacts.
The sequence length distribution of small RNA showed the highest abundance at 22 nt, indicating miRNA-like sequences were the most abundant sequences identified in the majority of samples, representing from 20 to 62% of total RNA sequence reads.
With more than a million copies scattered across the chromosomes, Alu elements, which run about 300 bases in length, are the most abundant sequences in the human genome.
For miR-21, the reference sequence was the most abundant sequence.
The most abundant sequence of each OTU was chosen as seed sequence.
Therefore, it was concluded that the most abundant sequence cluster represented putative clones of L. ochracea.
Additionally, we found that the most abundant sequence of miRNA-143 is not the reference sequence reported.
Gram-negative Bacteriodetes were the second most abundant sequences identified for both fresh and dry manure samples.
Members of Bacteriodetes and Actinobacteria were the second most abundant sequences identified from dry and fresh manure samples, respectively.
In 77 cases, this most abundant sequence did not correspond exactly to the current porcine miRBase 16.0 reference sequence (Table S10).
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