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A duty cycle in the data-dependent acquisition mode included a single MS scan followed by MS/MS scans on the three most abundant precursor ions (processed in reverse order of abundance) not subject to pre-determined or dynamic exclusion.
MS data was acquired using a data-dependent top10 method, dynamically choosing the most abundant precursor ions from the survey scan (300 1,800 m/z) for high-energy collisional dissociation (HCD) fragmentation.
A data-dependent procedure that alternated between one MS scan followed by 15MS/MS scans was applied for the 15 most abundant precursor ions above a threshold ion count of 20,000 in the MS survey scan with a following Dynamic Exclusion duration of 15 s.
Data were acquired using a data-dependent top10 method dynamically choosing the most abundant precursor ions from the survey scan (300 1800 m/z) for HCD fragmentation.
Each full MS (m/z range of 300 to 2,000) scan was followed by seven MS/MS scans of the most abundant precursor ions in the MS spectrum with dynamic exclusion enabled.
The three most abundant precursor ions were selected to be further isolated and fragmented.
Similar(11)
In the first run analysis, the 4 most abundant precursors were selected for MS/MS analysis.
A survey MS scan was followed by acquisition of MS/MS spectra of the five most abundant precursors.
LC-MS/MS experiments comprised cycles of five events; an MS scan with Orbitrap mass analysis at 30,000 resolution followed by CID of the five most abundant precursors.
Survey scans were acquired at a resolution of 70,000 at 200 m/z and the 15 most abundant precursors were selected for high-energy collision dissociation (HCD) fragmentation.
The ten most abundant precursors detected in the Orbitrap at 30000 resolution (2 4+ charge state) were selected for MS/MS in the LTQ Velos.
More suggestions(15)
most critical precursor
most abundant mineral
most common precursor
most likely precursor
most abundant animal
most obvious precursor
most volatile precursor
most abundant isotope
most abundant element
most abundant songwriter
most abundant compound
most infrequent precursor
most recent precursor
most abundant constituent
most abundant form
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