This study identified genes in the most abundant isolate of C. perfringens, so that the less abundant genotypes may not have been detected.
Accordingly, only two diatom strains (phylum Heterokontophyta) were successfully scaled up: Nitzschia sp. and Cyclotella sp. Cyanobacteria was the second most abundant phylum isolated by this method, representing 20% of the total isolates and included Pseudanabaena sp., Leptolyngbya sp. and Synechococcus sp. In order to isolate more cyanobacterial strains a different gating method will be needed.
By molecular techniques, the most abundant clones isolated from hypersaline microbial mats revealed to belong to S. strictum (as Acremonium strictum, Cantrell et al., 2013), indicating that Sarocladium species might have the ability to grow in environments with high salinity.
According to sequence analysis of the 16S rRNA genes, the most abundant bacteria isolated by culturing appeared to be the species of Bacteroides, which coincides with gut molecular diversity analysis [43].
However, Hahn and Mendgen [ 24] found that two of the most abundant genes isolated from their haustorial cDNA library encoded enzymes directly involved in thiamine metabolism.
In pig, saliva contains the pheromonal steroids 5α-androst-16-en-3-one 5α-androst-16-en-3-one 5α-androst-16-en-3-one 5α-androst-16-en-3-one 5α-androst-16-en-3-one 5α-androst-16-en-3-onelandd from submaxillary glands of mature males.
Bacillus was the most abundant genus (55 isolates), followed by Salimicrobium (4 isolates) and Marinococcus (3 isolates).
A single allele per locus per isolate (the only detectable allele, or most abundant allele in isolates which contain more than one genotype) was counted for the analysis comparing frequencies between severe and mild malaria groups.
The most abundant genotype amongst isolates from animals, t318 and t011, were not found in food (Tables 1 and 2).
The most abundant viral sequences isolated were from the Pepper mild mottle virus (PMMoV), with up to 109 virions per gram of dry weight fecal matter.
