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In this study, we found that, after RPA, DNA-PK is the most abundant factor in the HARP complex.
ATP, released into the extracellular space during cellular stress, is the most abundant factor activating NLRP3 [ 162], that is essential for processing pro-IL-1 β and secretion of IL-1 β [ 163], that, in turn, primes CD4+ and CD8+ lymphocytes and elicits the antitumor response.
Interleukin-6 (IL-6) was found to be the most abundant factor in the CAF secretome (Fig 6A, red square), as confirmed by ELISA on either CAF extracts (intracellular proteins) or CAF-CM (secreted proteins) which quantified about 1 ng/ml of IL-6 produced per 10 CAFs (Fig 6B).
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The theory on the putative role of Tβ4 in the physiological development of embryos, as well as in vascularization and tissue recovery in acute and chronic ischemia, has been reinforced by the discovery that Tβ4 is one of the most abundant factors secreted by embryonic endothelial progenitor cells [14].
Other reports have identified DNA-PK subunits as part of a group of potential cofactors for HARP,,, but our analysis reveals that RPA and DNA-PK are the two most abundant factors that stably co-purify with HARP (Fig. 1A).
Secondly, although the firing factors Dpb11, Sld2, Sld3 and Sld7 are not believed to play any role in elongation, nor to move with replication forks (Masumoto et al, 2000; Kanemaki & Labib, 2006), we identified them as amongst the most abundant factors associated with pre-RCs after DDK treatment and in fact are almost as abundant as pre-RCs even after 30 min in the presence of NTPs and dNTPs.
σ70 (RpoD) was the first discovered sigma factor [17] and is the most abundant sigma factor in E. coli, responsible for the transcription of genes needed for normal growth.
The MYB superfamily is one of the most abundant transcription factor (TF) families in plants.
The most abundant transcription factor found was classified under the C3H family.
Fibrinogen is the most abundant coagulation factor and the first one in reaching critical low levels during severe bleeding [ 1].
The second most abundant transcription factor found was MYB followed by NAC, WRKY, bZIP and AP2-EREBP family (Table 4).
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