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Cold-immobilized mosquitoes were secured in a vacuum saddle as previously described [ 45] and injected in the cervical membrane with approximately 20 D. immitis microfilarae (mf) in physiologic saline [ 46], and returned to the insectary for 24 or 48 hours prior to harvesting.
Similar(59)
loan is secured.
Al Mulahi was secured.
Informed consent was secured.
Individual lots of 14 mosquitoes were given the opportunity to feed on each animal, then transported to a secure laboratory.
Flies and mosquitoes were everywhere.
Mosquitoes were identified through morphological characters73.
Mosquitoes were embedded in Paraffin.
Exactly 30 mosquitoes were dissected per child.
All mosquitoes were tested only once.
Control mosquitoes were injected with dsLacZ.
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