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Circumsporozoite protein enzyme-linked immunosorbent assay was protein enzyme-linkedophelimmunosorbents out of which 13 mosquitoes were positive in CSP–ELISassay
Following feeding each mosquito was tested via real-time RT-PCR [34] to confirm infection status −100% of mosquitoes were positive with equivalent and high viral loads.
pipiens mosquitoes were positive for SINV RNA.
In Kranji Way, 7 (9.1%) of 77 female Ae. albopictus mosquitoes were positive for CHIKV, whereas 6 (13.5%) of 45 mosquitoes were positive in the Bah Soon Pah Road area.
quinquefasciatus mosquitoes were positive for WNV RNA, as detected by real-time reverse transcription PCR (RT-PCR) (7 ).
A total of 12 pools of Cx. p. pipiens mosquitoes were positive for WNV, which was confirmed by nucleotide sequencing.
Similar(51)
One pool of Cx. pipiens captured in the middle of August 2006 from Delta del Llobregat, in a typical Mediterranean climate that contained 3 female mosquitoes, was positive for flaviviruses.
Comparison of positive and negative mosquitoes for circumsporozoite protein detection by ELISA showed that mosquitoes that were positive for P. falciparum sporozoites had a higher L1014S kdr allelic frequency (Fst = 0.006; P = 0.033).
In conclusion, we found mosquitoes that were positive for D. repens DNA at a latitude of 52°N in northern Germany; these mosquitoes, from 3 species, were collected at 2 locations in 2011 and 2012.
arabiensis mosquitoes collected were positive for R. felis, including 1 male, suggesting transovarian transmission.
None of 12 pools of Cx. tarsalis mosquitoes assayed were positive for WNV RNA.
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