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All of the hybridizations (chloroquine-treated mosquitoes vs. untreated mosquitoes or chloroquine-treated infected mosquitoes vs. untreated infected mosquitoes) were performed twice.
Most of the studies of oral infectivity of CHIKV in Ae. aegypti and Ae. albopictus mosquitoes were performed using eGFP-expressing viruses.
Western blots of early phase (AeET) and late phase (AaSPVI, AaSPVII, AaLT) protease expression in unfed and blood fed dsRNA injected mosquitoes were performed as previously described [31].
Bioassays on the field-collected mosquitoes were performed in the CREC laboratory, in which temperature was maintained at approximately 20±2°C during the day, and at 26±1°C during observation periods (6 pm–8 am) with humidity >80% RH.
Collecting and rearing mosquitoes were performed as described previously [ 4].
DNA extractions on individual mosquitoes were performed following a rapid, simple alkaline method [ 35].
Similar(48)
From May through October 2006, monitoring of flaviviruses in mosquitoes was performed in the northeast region of Spain (Catalonia).
Collection of adult mosquitoes was performed indoors with pyrethrum spray catches and outside with US Centers for Disease Control and Prevention CDCC) light trap and oral aspirators (for hand catches).
Injections of 69 nL of liquid per mosquito were performed intrathoracically by using a Nanoinjector (Drummond) equipped with a sterile, finely drawn glass capillary needle.
Recently, rhythmic expression profiling of the Ae. aegypti mosquito was performed in a similar manner to our An.
Mosquitoes captures were performed once monthly from January 2004 to December 2006 in Candelária, and from October 2006 to December 2007 in Teotônio.
More suggestions(15)
mosquitoes were dissected
mosquitoes were transferred
mosquitoes were offered
mosquitoes were embedded
mosquitoes were controlled
mosquitoes were killed
mosquitoes were used
mosquitoes were tested
mosquitoes was performed
mosquitoes were reared
mosquitoes were released
mosquitoes were counted
mosquitoes were found
mosquitoes were blown
mosquitoes were held
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