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In another experiment, done with mosquitoes that weren't pretreated with antibiotics, Csp_P-fed mosquitoes were given blood containing the dengue virus and Plasmodium falciparum, a single-celled parasite that causes the most deadly type of malaria.
Individual lots of 14 mosquitoes were given the opportunity to feed on each animal, then transported to a secure laboratory.
Thirty males from the standard colony were introduced to the cage, and the mosquitoes were given 5 days to mate.
Where necessary, additional mosquitoes were given the opportunity to feed until the required number of successful bites was achieved.
Throughout the experiment, the mosquitoes were given access to sugar (with a cotton ball soaked with a saturated sugar solution), except for the day before blood feeding.
Blood-engorged mosquitoes were given 5% sucrose for maintenance, and the 0.2-L cages were placed inside larger cages and incubated as described.
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The new model for the transmission between humans and mosquitoes is given in the flowchart (Figure 1). Figure 1 Flowchart of the transmission of Dengue virus transmission between mosquitoes and humans.
The survival of mosquitoes is given by the equation: p = exp[ 1/(–4.4 + 1.31 T – 0.03 T )], [3] where T is the daily average air temperature in degrees Celsius (Martens 1997).
Mosquitoes then were given access to water and sucrose until 4 hr before blood-feeding.
All volunteers consented to mosquito capture and were given free malaria prophylaxis.
Associate Prof Simon Reid, a global disease control expert with the University of Queensland's faculty of medicine and biomedical sciences, said a future Zika virus outbreak in Australia was not impossible given that dengue mosquitoes were present in tropical northern Australia.
More suggestions(15)
mosquitoes were involved
bugs were given
mosquitoes were derived
mosquitoes were available
mosquitoes were dissected
mosquitoes were transferred
mosquitoes were offered
mosquitoes were embedded
mosquitoes were incriminated
mosquitoes were controlled
mosquitoes were killed
mosquitoes were used
mosquitoes were treated
mosquitoes were tested
mosquitoes were sorted
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