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To test the relative fitness of their transgenic mosquitoes in the context of Plasmodium infection, the researchers conducted a series of experiments in which initial populations that were made up of equal numbers of wild-type and transgenic mosquitoes were allowed to interbreed and expand for 13 generations.
For mosquito infection, A. stephensi mosquitoes were allowed to bloodfeed on anesthetized mice for 15 minutes.
Mosquitoes were allowed to feed for 45 minutes before the blood meal was removed.
The mosquitoes were allowed to feed on the mice for 20 minutes without interruption.
Once restrained, mosquitoes were allowed to equilibrate to 24°C and the necessary manipulations were performed (see below).
Adult mosquitoes were allowed access to a cotton pad soaked in 10% sucrose solution as a carbohydrate source.
Similar(20)
Mosquitoes are allowed to develop oocysts before being dissected.
Immature mosquitoes are assumed to be sedentary, while adult mosquitoes are allowed to move between patches.
An additional cohort of mosquitoes was allowed to salivate for intervals (repeated in triplicate) to duplicate times of observed mosquito feeding.
On August 27, 1900, an infected mosquito was allowed to feed on Carroll, and he developed a severe attack of yellow fever.
The ventral surface (primarily abdomen) of each mouse was placed on a screened cardboard container harboring sporozoite-infected female A. stephensi mosquitoes, which were allowed to feed for 20 minutes.
More suggestions(15)
mosquitoes were offered
mosquitoes was allowed
mosquitoes were embedded
mosquitoes were controlled
mosquitoes were killed
mosquitoes were used
mosquitoes were tested
mosquitoes were reared
mosquitoes were counted
mosquitoes were released
mosquitoes were found
mosquitoes were held
mosquitoes were blown
mosquitoes were discarded
mosquitoes were maintained
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