Sentence examples for mosquitoes using a from inspiring English sources
Total genomic DNA was isolated from individual mosquitoes using a standard phenol-chloroform extraction procedure after proteinase K digestion.
About 69 nl of dsRNAs (207 ng of dsRNA) in water was introduced into the thorax of cold-anesthetized 1-day old (for systemic silencing) or 3 4 days-old (midgut silencing) female mosquitoes using a nano-injector (Nanoject; Drummond Scientific, Broomall, Pennsylvania, United States with a glass capillary needle according to established methodology [28].
Total DNA was extracted from mosquitoes using a salt extraction and ethanol precipitation procedure [ 12].
DNA was extracted from individual mosquitoes using a CetylTrimethylAmmonium Bromide (CTAB) [ 27] or phenol/chloroform protocols [ 28].
Total RNA was extracted from batches of 10 three-day old adult female mosquitoes using a PicoPure™ RNA isolation kit (Arcturus) according to manufacturer's instructions.
We also identified six more Y chromosome genes in Anopheles mosquitoes using a more efficient approach called the chromosome quotient (abbreviated CQ) (Hall et al. 2013).
To verify the transcription of AaIT gene in Bb-AaIT, the total RNAs were extracted from the CDA culture supernatant or infected mosquitoes using an RNeasy mini plant kit (Qiagen, Duesseldorf, GER), and they were subjected to reverse transcription with random primers and then PCR with the primers RT-AaIT-F and RT-AaIT-R (5′-CTTTCTTCGGTTCTCGCCCT-3′ and 5′-CCTTATCGGCGTAGTGGACC-3′, respectively).
In Spain, residents can flag the presence of tiger mosquitoes using an app called Atrapa el Tigre (Catch the Tiger).
Vector competence which refers to the intrinsic permissiveness of a vector to transmit a pathogen is measured in laboratory by estimating oral susceptibility of mosquitoes using an artificial feeding protocol.
If this pathway is blocked by the E2-211I mutation then CHIKV may infect Ae. albopictus mosquitoes using an alternative receptor(s) that targets virus into different endosomes, in which the presence of the E1-A226V does not result in differential infectivity.
Collectors were trained to capture mosquitoes using an aspirator and a flashlight while inside the nets.
