Mosquitoes were placed on a refrigerated table for species or taxonomic group identification before being placed in pools of <200 mosquitoes and sent to Queensland Health Forensic and Scientific Services or the University of Queensland, Brisbane, for JEV detection.
The mosquitoes were placed on exposed forearms and allowed to feed for 5 minutes, after which they were removed and dissected to determine whether the mosquito had 1) fed and 2) salivary gland sporozoites.
After collection, the mosquitoes were placed on dry ice for transport back to the Medical Entomology Laboratory, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, Monterrey, where they were separated into pools of ≈10 insects each, based on species, date, and method of collection (Table 1).
It's a rainy afternoon in South Beach and tourists who have braved the weather to eat at the Miami Grill on Washington Avenue are helping themselves to sprays of mosquito repellent placed on the counter by staff.
In short, 3 5 days-old female mosquitoes (30 40 mosquitoes per assay) were placed on a two-choice arena designed to attract host-seeking mosquitoes.
Six days post inoculation of the parasites, when parasitaemias were ∼20% and the presence of gametocytes of both sexes was confirmed microscopically, mice from each mixed strain infection group were anaesthetised and placed on a mosquito cage, each containing ∼250 female mosquitoes.
In assay 3, 30 anesthetized female mosquitoes in each group were placed on the 50 cm2 filter paper that had absorbed 3 ml of conidial suspension at 1 × 105 conidia/ml (low), 1 × 106 conidia/ml (middle) and 1 × 107 conidia/ml (high) or 0.02% Tween 80 (control) for 10 s.
No feed remained uneaten when occasionally the daily ration of feed was placed on a feed tray made of mosquito net and steel frame, and placed on the pond bottom.
Supernatant from the 10% brain homogenate was placed on monolayers of A. albopictus C6/36 mosquito cells in cell culture tubes.
We saw a ball of elephant dung and were told that when coals are placed on it, the fumes help to keep mosquitoes away.
