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Exact(6)
These particular children then went on to be significantly more likely to infect mosquitoes at day seven after treatment, and also more likely to be one of the minority (about 10%) that again had parasite positive blood slides under the microscope 28 or 42 days after treatment, once the malaria drugs have been completely cleared from the body.
The entire MG was dissected from more than 1500 mosquitoes at day 5 after egg hatching.
Mosquito midguts and abdominal walls containing fat body tissues were collected from pools of 40 sibling mosquitoes at day 1 and on day 11 after the blood meal.
A total of 5,726 (38.4%), 4,687 (31.5%) and 6,624 (44.5%) transcripts were expressed differently in blood-fed mosquitoes at day 10, 19 and 28 post emergence, respectively, when compared to sugar-fed mosquitoes.
The influence of the number of mutations in infections at enrolment on the proportion of infected mosquitoes at day 14 was tested using logistic regression models with Generalized Estimating Equations (GEE); the influence of the number of mutations in infections at enrolment on oocyst densities in mosquitoes was determined using negative binomial regression.
Significant differential expression is defined as P≤0.001 as the cut-off criterion after correction for multiple testing using the bloodfed group as mosquitoes at day 1 post emergence showed the largest difference from other age groups in gene expression profiling.
Similar(54)
One cage (HD standard conditions) was excluded from the proliferation analysis because only one of the ten mosquitoes dissected at day 14 was infected, and only with a single oocyst, suggesting that the infection failed to transmit from this host.
However, no DENV-2 was detected in mosquito saliva at day 10 pi.
(i) We assessed the intensity of infection by examining the presence of oocysts in mosquito midguts at day 10.
TR and TE were mostly similar among all three mosquito populations at day 14 (Chi square test: p > 0.05).
Eighteen immunized volunteers, plus six unimmunized infectivity controls, were challenged with P. falciparum (strain 3D7) via five infectious mosquito bites at day 28.
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