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The city plans to conduct ground spraying with the pesticide Anvil in seven neighborhoods of central and western Staten Island, areas where the virus-infected mosquito samples were found, between 10 p.m. Saturday and 5 a.m.
Mosquito samples were collected from these sampling sites by trained field assistants (who were postgraduate research students).
Of these, close to 500 mosquito samples were collected from the Bapunagar area in Ahmedabad, where three of the positive cases were eventually found.
The adult mosquito samples were collected at dawn and dusk, as this is the time when mosquitoes are highly active and are found abundantly.
In this spatiotemporal study, 1,156 mosquito samples were collected from 50 study sites with unique ecological characteristics within the city and a taxonomical exercise was conducted to investigate different vector species thriving in different months and seasons of the year.
Groups of ten mosquito samples were collected at different developmental stages: fourth-instar larvae, early pupae (pre-tanning stage), late pupae (tanned stage), and adult mosquitoes (male and females).
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RVFV RNA in mosquitoes collected within the area was high, and the substantial presence of WNV RNA in these mosquito samples was unexpected.
Actually, vector competence of the tested Argentine and Uruguayan mosquito samples was similar to those of several Ae. aegypti populations sampled in dengue epidemic Brazilian localities tested for the same DENV-2 strain previously [ 22].
Every year, mosquito sampling was performed biweekly from 1 May through 31 October by FCDHW staff.
Mosquito sampling was conducted only at homesteads where specific animals, those known to be reservoirs of RVFV, were housed closely with humans.
Mosquito sampling was conducted in 2 areas within Masalani Division, Ijara District, North Eastern Province, where human surveillance had taken place 8 months before the RVF outbreak (2 ).
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