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Then they compared mosquito genomes and narrowed the search with a gene they named APL1.
Four families have elements in two mosquito genomes and three families are distributed in the three Nasonia wasps.
This is consistent with the interpretation that Juan has been retrotransposing in mosquito genomes, and this region is under negative selection due to functional constraint.
Alignments for the different subclasses were constructed with sequences from all the three mosquito genomes and in the case of Classic subfamily, along with Drosophila sequences.
The homologous sequences plus 200 bp flanking sequences were retrieved from three mosquito genomes and aligned using Clustalx [ 38] with a gap open penalty of five and a gap extension penalty of 0.05.
A bootstrap consensus tree was constructed using the neighbor joining method (Saitou and Nei 1987) with all the Classic OBPs from the three mosquito genomes and the D. melanogaster with 1000 bootstrap replicates (fig. 4).
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Advances in genetic engineering technologies make it possible to introduce foreign DNA into the mosquito genome and either decrease the lifespan of the mosquito, or make it more resistant to the malaria parasite.
To understand the complexity of this family in insects, we analyzed FREPs in the mosquito genome and made comparisons to FREPs in the fruitfly genome.
Thirty millions 20 bp cDNA tags were sequenced, mapped to the mosquito genome and clustered, representing 6850 known genes and 4868 additional clusters not located within predicted genes.
For comparative analysis, we also identified 68 ABC genes from the flour beetle, Tribolium castaneum (Coleoptera), 52 from A. gambiae (8 more compared to 44 in [ 4] due to the updated mosquito genome), and 43 from the honeybee, Apis mellifera (Hymenoptera) (Table 1).
Although several studies have been performed on codon bias patterns in mosquito genomes (Behura and Severson 2011, 2012b; Rodriguez et al. 2012), no research has been conducted to understand how codon bias relates to intron loss or gain of genes in mosquitoes.
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