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The cuticular proteins may play a role in insecticide resistance by limiting the penetration of insecticides into the mosquito cuticle.
For example, [ 19] demonstrated that a mosquito cuticle protein (AAEL011045) binds to a viral envelope and thus inhibits infection.
The moth genes in the clade containing the mosquito cuticle protein AAEL011045 are all candidate immune system genes that may be able to bind to the envelope of PiGV and thus inhibit its infectivity.
In order to discover if the degree of sequence identity amongst the 7 differentially expressed cuticle-proteins in this study could be used to explain their opposing pattern of regulation (i.e. up versus down), we aligned them with mosquito cuticle protein AAEL011045 in BioEdit v.7.2.4 [ 20] and built a neighbour-joining tree using CLUSTAL v1.2.0 [ 21].
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3 5 day old female mosquitoes (n = 30) were removed from the colony, knocked down at −20°C, washed in ice-cold 95% ethanol to overcome the hydrophobic properties of mosquito cuticles and rinsed in ice-cold water, and then submerged in RNAlater (Qiagen, USA) and frozen at −80°C.
It is possible that thicker mosquito cuticles slow insecticide absorption and consequently increase the efficiency of metabolic detoxification [ 21].
Bioassays for the pathogenicity of the Bb-AaIT and WT against the 2-instar larvae and the female adults of Aedes albopictus mosquitoes (through cuticle penetration or conidia ingestion) were conducted at low, middle, and high conidial concentrations.
(b, c) 3-day-old (3 days after emergence) female adult mosquito infection through cuticle contact (b, assay 2) or through conidia ingestion (c, assay 3).
The virulence of Bb-AaIT and the wild type to larval (with infection through conidia ingestion) or adult mosquitoes (infection through cuticle or conidia ingestion) were compared at low, middle and high concentrations by survival analysis.
In other words, the Bb-AaIT strain was capable of killing the larvae by ingesting the conidia in suspension and killing adult mosquitoes through both cuticle infection and conidia ingestion at a higher efficiency than the wild-type strain.
The yellow gene in Drosophila is responsible for tanning of the cuticle, and the mosquito homolog was shown to have a dopachrome oxidase function [ 53, 54].
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