In control embryos by injected linearized donor plasmid alone, only 20.2% of embryos showed weak and high rates of the mosaic expression of RFP and few fish expressed RFP at the 200-d-old stage (data not shown).
Migration of caRhoA-Expressing Stable-Bleb Cells In Vivo, Related to Figure 7 (I-II) Two-photon time-lapse movies of transformed stable-bleb cells migrating in the 3D tissue environment of Tg (actβ1 myl12.1eGFP ) transgenic embryos with mosaic expression of caRhoA plus GPI-RFP (membrane, red) to mark caRhoA expressing cells.
Nonetheless, detection of H3K27me3 on a subset of expressed genes in the ICM also suggests that these genes are not expressed in all cells of the ICM, in agreement with the mosaic expression of many genes in the ICM [1], [27].
We initially believed that the mosaicism mediated by the CamKII-Cre transgene was caused by low – rather than mosaic – expression of the Cre recombinase, translating into infrequent (stochastic) activation of Cre-dependent transgenes.
We observed mosaic expression of mCherry in all sections (10%-90 10%-90lls, Figure 1B and Supplementary information, Figure S2A and S2B).
Due to the mosaic expression of Cre recombinase, MuSK is not globally inactivated and new synapses are formed aberrantly patterned across the diaphragm.
These lesions also showed mosaic expression of SNF5 by immunohistochemistry. Therefore, our studies implicate that modest changes in angiogenic regulation interact with perturbations of SWI/SNF complex activity to modulate disease phenotypes.
Moreover, we have previously shown that injection of just a few cells in the embryo (for example, with depolarizing reagents) is sufficient to cause complete conversion21,25 the partial conversion here is not due to mosaic expression of the CREB construct, as injected alone, in one cell of the 32-cell stage embryos as above, causes an incidence of ~45% complete conversion.
The mosaic expression of growth factors during skeletogenesis indicates that diverse bones have different expression of them and it should have therapeutic significance [[5]].
Rett syndrome is an autism spectrum disorder caused by mosaic expression of mutant copies of the X-linked MECP2 gene in neurons.
The mosaic expression of such features in early Homo, including Homo habilis, Homo rudolfensis, and some early H. erectus, suggests that these traits do not constitute an integrated package.
