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Plasmid pEB123 contains all the parts of TAGIT except for the ME (hyperactive mosaic end) that are recognized by the Tn5 transposase.
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(1) At either end of TAGIT are the optimized minimal inverted repeats (19 bp mosaic ends; ME) that allow the hyperactive Tn5 transposase to mediate transposition [32].
The mosaic ends (MEs) that define the hyperactive Tn 5 transposon [ 9] have two possible open reading frames (ORFs) through them.
The construct was adapted for use in S. aureus by the addition of mariner mosaic ends (ME) and an erythromycin resistance gene.
Either side of the locked gates of the car park a lion sits proudly, one paw raised, with the beautiful Holte End mosaic in the background.
Epstein said that reading the book is like "looking at a mosaic very close up, tile by tile", and that the big picture only emerges near the end.
Mr. Hawkins ended up furnishing the St . Regissuite with his mosaic bed and other furniture.
Mosaic organisation of chromosome ends suggests that EXT repeats are more susceptible to DSB.
At the far end, a blue mosaic plinth sits like an island in the clear water.
The recognition of tobacco mosaic virus (TMV) since the end of nineteenth century [1] has sparked innumerable research towards its potential applications in biomedicine [2, 3] and biotemplates for novel nanomaterial syntheses [4, 5].
All rescued clones contained a single copy of the transposon with intact mosaic element sequences at each end that were flanked by the 9-bp insertion site sequence duplications typical of EZ::TN transposase.
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