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Genetic mosaic analysis of Rheb mutant photoreceptor projections showed the same phenotypes, demonstrating that normal levels of Tor-Tsc signaling in the retina are required for proper photoreceptor targeting (data not shown).
As a more direct test, we conducted genetic mosaic analysis of kin-1 in an spr-5 mutant background.
The cell-autonomous model is further supported by the observation that mosaic analysis of Dsh MO-injected embryos gave results similar to those of Wnt5α disruption.
To test more definitively whether acy-4 (ok1806 ) sterility requires twk-1 function in the somatic gonad, we conducted genetic mosaic analysis of acy-4 (lf) suppression.
For genetic mosaic analysis of inx-8 inx-9 using DG3614, we did not formally screen for losses of the rescuing array in the germline lineage.
For genetic mosaic analysis of the sacy-1 (tm5503 ) gamete degeneration phenotype, we used sacy-1 (tm5503 ); unc-119 (ed3 ); tnEx159 [gfp:: sacy-1 unc-119 ].
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Our mosaic analysis on SNP data from a salivary sample identified three mosaic events on chromosome 18, two deletions and one duplication in ∼50% of cells.
In this study, resting on our characterization of multiple Smr mutant lines, mosaic analysis with a loss-of-function Smr allele, and knockdown experiments with two Smr.IR fly lines, we report the involvement of SMRTER in the development of ovarian follicle cells and wing.
To confirm the cell autonomous requirement for Pacman in wing disc cells we used mosaic analysis where clones of cells with a mutant or wild-type homozygous genotype are induced in a heterozygous background by mitotic recombination.
This type of mosaic analysis has been used to provide evidence of direct activation of SNB soma size and gene expression via actions on motoneuronal Ar, and also that masculinization of SNB motoneurons occurs independently of motoneuronal Ar (Table 1).
An advantage of mosaic analysis in the zebrafish over other model organisms is that the development of the embryos occurs ex utero and the embryos are transparent.
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