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Chromosomes that contain FRT sites can be induced to undergo mitotic recombination, and hence are widely used for performing mosaic analysis in Drosophila.
Collectively, these studies provide several new tools for genetic mosaic analysis in the Drosophila ovary.
The lines we have described here complement existing tools for genetic mosaic analysis in Drosophila (Hafezi and Nystul 2012).
Here, we show that the random activation of genes through microsatellite instability can be a valuable tool for mosaic analysis in zebrafish.
An advantage of mosaic analysis in the zebrafish over other model organisms is that the development of the embryos occurs ex utero and the embryos are transparent.
Mosaic analysis, in which two or more populations of cells with differing genotypes are studied in a single animal, is a powerful approach to study developmental mechanisms and gene function in vivo.
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Genetic mosaic analysis used in this study showed that odor responses are normal in the absence of Gαq, which is required for normal CO2 responses [30].
There is a rich history of scientists studying gene functions using mosaic analysis, particularly in Drosophila.
A schematic summary of the mosaic analysis is shown in Figure 4A.
Historically, the first method to be used was mosaic analysis, widely applied in Drosophila and C. elegans.
In mosaic analysis, the wild-type allele of a gene is removed in a heterozygous individual only in particular tissues; upon subsequent mitosis, there are patches of cells that have only the mutant allele for the gene.
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