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In the post mortem lungs of Patient 1, the major histopathological finding was diffuse alveolar damage (DAD), reflecting the clinical syndrome of acute respiratory distress syndrome (ARDS).
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There is evidence that elimination of crocidolite asbestos occurs (Berry, 1999), and from regression analysis of post mortem lung content of crocidolite fibres on years since last exposure in 90 former Wittenoom workers, a clearance rate of 9% a year was estimated after allowing for exposure (de Klerk et al, 1996).
PAMs were obtained by post mortem lung lavage of 8-week-old PRRSV free pigs, and seeded into 96-well plates for 24 h incubation till further assays in RPMI-1640 supplemented with 10%% fetal calf serum (FBS), 2 mM L-glutamine, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate and a mixture of antibiotics.
This data was confirmed in the endothelium of post-mortem lung tissues of SCD patients.
Retrospective analysis of post-mortem lung tissue of individuals that died from the 1918 pandemic influenza strain indicated that most of these people also had a bacterial infection.
TRALI was defined by the development of both hypoxaemia either during or within two hours of the second event transfusion, and pulmonary oedema upon blinded histological assessment of post- mortem lung sections.
Aspergillus flavus grew from a post mortem lung culture from one patient who died of respiratory failure.
Post mortem, lung tissue was harvested for the determination of the wet/dry weight ratio and airway obstruction.
We found that pre mortem lung function determinations had no impact on any of the above parameters.
In Patient 1, post mortem lung, lymph nodes, spleen, liver, distal small bowel and kidneys were also examined for presence of swine-origin H1N1/09 viral RNA.
Post mortem lung sample from Patient 1 was stored in paraformaldehyde and embedded in paraffin for haematoxylin and eosin staining.
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