Sentence examples for mortar in liquid from inspiring English sources

Then, 200 mg of leaf material was powdered in a precooled mortar in liquid nitrogen.

The frozen samples (about 2 g) were ground in a mortar in liquid nitrogen and placed in 10-mL centrifuge tubes in ice.

The frozen bone biopsies were pulverized with a mortar in liquid nitrogen with their content of marrow intact.

Samples were ground in a mortar in liquid N2 then in 2×1 mL extraction medium consisting of 80% methanol containing 100 µM Ribitol as internal standard.

Skin equivalents were grinded with a mortar in liquid nitrogen and lyzed in 0.5× PBS containing 1% Igepal CA-630 (Sigma-Aldrich) and a protease inhibitor cocktail (P8340, Sigma-Aldrich) at a dilution of 1∶500.

To extract bone proteins, calvaria and femur were ground in a mortar in liquid nitrogen followed by sonication in 50 mM Tris-HCl buffer, pH 7.5 containing 150 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, 1% Triton X-100, and protease inhibitors (Roche).

After frozen tissue was pulverized in a mortar cooled in liquid nitrogen, RNA extraction was accomplished using the peqGOLD TriFast™ reagent (PEQLAB Biotechnologie GmbH, Erlangen, Germany).

At the time of study, tissue samples were broken into smaller fragments in a mortar cooled in liquid nitrogen, and individual fragments were processed directly for RNA extraction.

For each animal analyzed, a piece of frozen liver tissue was ground to fine powder in a mortar precooled in liquid nitrogen.

The liver samples were pulverized in an aluminum mortar precooled in liquid nitrogen.

Lysate was produced by grinding the frozen slurry with a pestle in a 35-mm diameter mortar suspended in liquid nitrogen until a fine powder was obtained.