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Mean values established using Diff-Quick for the main morphometric variables were: head length 2.93 ± 0.13 μm; head width 2.33 ± 0.15 μm and tail length 34.16 ± 1.66 μm.
However, other possible criteria, such as nauplii and PL1 biochemical and morphometric variables, were not reliable as predictive indicators under our experimental conditions.
On each experimental handaxe, 29 size-adjusted (scale-free) morphometric variables were recorded to capture the overall shape of each handaxe in order to compare them statistically to the model.
Morphometric variables were computed using direct, three-dimensional techniques that do not rely on assumptions about the underlying structure.
Morphometric variables were determined by image analysis, using an IBM computer (IBM Computer, Inc.; Armonk, NY).
Morphometric variables were determined by labeling lymphatic vessels with anti-human D2-40 and blood vessels with anti-CD34 antibody.
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This test was used because gender is a binary qualitative variable, and the morphometric variables are normally distributed quantitative variables.
Leaf length of Capsicum plants from Santiago increased as relative humidity increased suggests that high morphometric variables are not necessarily related to environmental conditions, since leaf length values were higher in those plants from Los Gatos, where relative humidity was the lowest compared to the other sites.
Instead, the pattern of variation in male tail length was more similar to those of morphometric traits, where environmental variables were retained as important predictors of variation, but geographic distance was not.
For each morphometric trait, different combinations of environmental variables were retained as important predictors, resulting in markedly different patterns of spatial variation (see Figures 2a and b for examples of predicted variation in morphometric traits).
The morphometric analysis was focused on selected pathological areas where two variables were measured: the labelling index (LI) and the mean optical density (MOD).
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