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The Image-pro morphometric software was then used to measure the area of each of these colonies.
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ARC GIS software was utilized for morphometric component analysis and delineation of the watershed using SRTM digital elevation model (DEM).
The Olympus CellSensDimension software was used for morphometric analysis.
For morphometric studies Image Pro Plus (Media Cybernetics) software was used.
For the morphometric analysis, the Image Processing and Analysis in Java, Image J software was used.
Following immunofluorescent detection of insulin and glucagon, Metamorph morphometric software (Universal Imaging) was used to measure the approximate area on each section occupied by either insulin (green channel) or glucagon (red channel) by manual thresholding of pixel intensity.
In order to assess OXTR expression, a quantitative immunohistochemistry based on morphometric software (Quantimet 500C+) was performed twice by two independent researchers.
Hence, nuclear morphometric measurements made using image analysis software are deemed to be an objective and reproducible tool in anticipating the survival of patients.
The area of the gel was measured using morphometric software (WinRoof; Mitani), and the contraction rate was obtained by the division of the gel area with CCCM stimulation by the initial gel area.
Thereafter, quantitative immunohistochemistry based on morphometric software (Quantimet 500C+, Leica, UK) was applied for CX3CR1 receptor identification in paraffin-embedded 5 μm sections of the HUVECs cultures under light microscopy.
To blind the interpretation to cytologic features, nuclei and cytoplasm were obscured using morphometric software (Zen 2011, Carl Zeiss Microscopy, Germany).
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