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Variables of bone architecture (volume fraction, trabecular number, thickness, and separation, degree of mineralisation, and degree of structural anisotrophy) were calculated with routine morphometric software.
To blind the interpretation to cytologic features, nuclei and cytoplasm were obscured using morphometric software (Zen 2011, Carl Zeiss Microscopy, Germany).
The percentage of area occupied by necrosis was estimated using the "Olympus DP-Soft" morphometric software.
The Image-pro morphometric software was then used to measure the area of each of these colonies.
Semi-quantitative evaluation of single positive cells (pro-SP-C) and double positive cells (pro-SP-C+N-cadherin) was perfomed using the Axiovision morphometric software.
The body-shapes of the tadpoles were captured by digitizing 12 landmarks (Fig. 1) on each image, using the geometric morphometric software TpsDig v1.40 [31].
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Interstitial fibrosis was quantified using a computer-based morphometric analysis software (Analysis, Olympus) that allowed the formation of a binary image in which the stained area could be automatically calculated as percentage of the image area.
These were done by processing and calculating the stained image using ImageJ [ 28] 1.47 morphometric computer software (Wayne Rasband, National Institute of Health, USA).
The ratio of villi length over crypt depth was measured by Image-Pro Plus 5.1 morphometric analysis software (Media Cybernetics, Inc. Rockville, MD).
The present study assessed the new malignancy grading system at tumor proper (TP) and Bryne's grading system at invasive tumor front (ITF), morphometric features using software, to clarify their associations with prognosis of oral cancers.
Morphometric analysis using ImagePro software demonstrated that this change in collagen deposition was statistically significant (P < 0.05).
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