Sections were overlaid with a morphometric grid and the area of myelin loss and loss of astrocytic antigens was manually determined by counting the grid points over the respective areas.
Cell densities (neurons per cubic millimeter) were estimated in 8 μm Neu-N-stained slices at 400x magnification with a morphometric grid methodology using Abercrombie's correction [ 36] as previously described and well established in the literature for surgical hippocampal fragments [ 1, 3, 8, 11, 37– 41].
Cell densities (neurons per cubic millimeter) were estimated in 8 μm Neu-N stained slices at 400x magnification with a morphometric grid methodology using Abercrombie's correction [ 50] as previously described and well established in the literature for surgical hippocampal fragments [ 3, 5– 7, 51– 51].
Cell densities (neurons per cubic millimeter) were estimated in 8-µm Neu-N stained slices at 400× magnification with a morphometric grid methodology using Abercrombie∼s correction (Abercrombie, 1946) as previously described (Babb et al., 1984a; Mathern et al., 1995a; Kandratavicius et al., 2012).
Quantification was done by using a morphometric grid.
The area of meninges was determined using a morphometric grid as described in detail before (Dal-Bianco et al., 2008).
All histological measurements and cell counts were made using standardized microscopic fields defined by an ocular morphometric grid.
Counts of capillary and arteriolar density per square millimeter were obtained after superimposing a calibrated morphometric grid on each digital image using Adobe Photoshop Software.
For quantification, the immunostained sections were overlaid by a morphometric grid and inflammatory cell numbers or acutely injured or transected axons were manually counted in 5 100 fields per tissue area.
Averages were obtained by counting at least 6, and if possible, 10 standardised fields of view per lesion of 10 000 mm each defined by an ocular morphometric grid as previously described [ 25].
